Peptide Reconstitution Calculator

Reconstituting a lyophilized peptide? Enter the amount in the vial, the bacteriostatic water being added, and the target dose. The calculator returns the concentration, the exact line to draw to on a U-100 insulin syringe, and how many doses each vial yields.

How to reconstitute a peptide

1. Bring vials to room temperature. Let the lyophilized peptide vial and the bacteriostatic water sit until they reach room temperature.
2. Add the water slowly. Draw the chosen volume of bacteriostatic water and let it run gently down the inside wall of the vial, never inject it directly onto the powder.
3. Swirl, don’t shake. Gently swirl or roll the vial until fully dissolved. Shaking can damage the peptide.
4. Store correctly. Refrigerate the reconstituted vial at 2–8°C and use within its stable window. Store unreconstituted vials below 25°C, away from light.

Reading the result

On a standard U-100 insulin syringe, the barrel is marked 0–100 “units” (IU), where 100 IU equals 1 ml. The calculator’s middle figure is the unit line to draw to for the target dose at the concentration you mixed. Halving the bacteriostatic water doubles the concentration and halves the draw volume — useful when you want smaller, more precise draws.

All Aura Labs products are supplied for laboratory and research use only. They are not for human consumption, medical, or veterinary use. This calculator is provided as a planning guide and is not medical or dosing advice.